The joy (and bane) of having a model is that when new information arrives, you can see if it supports or disagrees with the model. In some cases, it become a neutral — not sufficient information to make a determination.

The National ME/FM Action Network did an announcement of three bio markers, from Australia, Canada and the United Kingdom (i.e. the British Commonwealth!)

• Canada: “These preliminary results suggest that IL-1α, 6 and 8 adjusted for illness duration may serve as robust biomarkers, independent of age, in screening for ME/CFS.”
• London: “Natural killer cells demonstrated the greatest changes in miRNA expression with up regulation of hsa-miR-99b and hsa-miR-330-3p. These findings are functionally consistent with current understanding of the pathogenesis of CFS/ME.” MicroRNAs hsa-miR-99b, hsa-miR-330, hsa-miR-126 and hsa-miR-30c: Potential Diagnostic Biomarkers in Natural Killer (NK) Cells of Patients with Chronic Fatigue Syndrome (CFS)/ Myalgic Encephalomyelitis (ME) [2016].
• Australia: ” the first to identify circulating miRNAs from ME/CFS patients. They found three distinct miRNAs that might serve as biomarkers.”

Testing against the model

• IL-1 alpha, IL-6, IL-8  – all of these are documented to be effected by probiotics
  • “but a combination of infections followed by cell damage leads to release of the intracellular cytokine interleukin (IL)-1 alpha from endometrial cells, which then acts to scale inflammatory responses.” [2016]
  • “, feeding maize fibre and soyabean fibre raised IL-1α and TNF-α mRNA levels.” [2013]
  • “Lactobacillus rhamnosus GG (LGG).. inhibits activation of …interleukin-1alpha, or gamma-interferon.” [2002]
  • ” mechanism of probiotics by testing effects of IL-α, which is upregulated in the peripheral blood mononuclear cells of Enterococcus  faecium-supplemented piglets,” [2013]
  • ” lactobacillus [Reuteri] inhibited the ETEC-induced expression of proinflammatory transcripts (IL-6 and TNF-α) and protein (IL-6),” [2016]
  • “L. salivarius PS2 intake led to a reduction in milk bacterial counts, milk and blood leukocyte counts and interleukin (IL)-8 level in milk,” [2016]
• hsa-miR-99b and hsa-miR-330-3p
  • Only 4 articles:
    • The hsa-miR-125a/hsa-let-7e/hsa-miR-99b cluster is potentially implicated in Cystic Fibrosis pathogenesis.
    • Differential expression of miRNAs in esophageal cancer tissue [2013].
    • Microrna profiling analysis of differences between the melanoma of young adults and older adults.
    • All of the above are conditions associated with low Vitamin D levels, as is CFS.

Looking at miRNA

• “Our results suggest that 68 bacterial RNAs predicted from 37 different bacterial genomes have predicted secondary structures potentially able to generate putative microRNAs that may interact with messenger RNAs of genes involved in 47 different human diseases.” [2014] i.e. bacteria DNA is adopted into the human cells, producing miRNA.
• “a third mycobacterial species, M. marinum, did express an ∼23-nt small RNA that was bound by RISC and derived from an RNA stem-loop with the characteristics expected for a pre-microRNA.” [2014]

Bottom Line

One set of markers is strongly associated with microbiome shifts. The second with Vitamin D levels.  miRNA can cross from bacteria (including gut bacteria) into human cells. All of these tests appear consistent with the microbiome model

While the tests may help with getting a faster diagnosis, they do not appear to lead to treatments.

I have a friend in the 80’s who for years have been eating using food banks, a lot of prepared/microwaveable meals. Recently a change of circumstance resulted in him moving into a place where food is all made from scratch, often organic.  A lot of his lab tests went from concerning to healthy over 6 months. He does not have CFS, but this does illustrate that diet can have a very major impact. I know that many CFSers survive on food banks in various countries around the world.

Often, one of compounding problems is that a CFS patient is so tired, that all they can do is put a package into the microwave (which can reduce the vitamin and other good content by 50%). Even boiling vegetables in soft water, strips remove nutrients. Overcooked, does the same.

One of the irony that I see is that people will spend money on Inulin capsules, instead of using natural sources (and often bringing other minerals and vitamins with it), for example: Banana, Garlic, Chicory root, Globe artichoke, Jerusalem artichole, Jicama, Onion, wild yam [wiki]

I have posted individually in the past and will attempt to reduce this to a simple reference page without citation (see links below for citations)

What is my daily diet?

I do not keep religiously to it, but this is my ideal pattern

• Breakfast: Either rye bread with butter/ cheese/ and eggs OR porridge with bran added (Not pasturized milk or goat milk)
• Lunch: Crisp break or rye bread with cheese, a banana or other fruit
• Supper: 4 oz of fish or meat, salad (I like Kale) with nuts, one or two of the greens above
• Snacks: Mixed Nuts

References:

• “Compared with milk intake, cheese consumption significantly reduced urinary citrate, creatine, and creatinine levels and significantly increased the microbiota-related metabolites butyrate, hippurate, and malonate.” [2015]
• How do I get there from here!!!???!! — Part Two
• Sorbitol / Glucitol
• Diet Impact on Microbiome
• E.Coli and Green Vegetables
• Migraines, diet and the microbiome
• Roundup and food grown with Roundup
• Peanuts – A recommend part of diet
• Wikipedia FODDMAR list is in the right general direction but their target is different and their reported results to date has been less than ideal.

A reader asked if this might be suitable with my model. Information was sparse but a 2010 studies suggests that the harm aspect may be greater than the good.

Low dose naltrexone: side effects and efficacy in gastrointestinal disorders. [2010] [Full Text]

• 58 of 121 had one or more neurological complaints  – almost 50%
• In 13 patients with idiopathic irritable bowel syndrome, 2 were markedly worse.
• . In 85 patients with irritable bowel syndrome-small intestinal bacterial overgrowth, 15 were markedly improved, 32 were moderately improved, 11 were mildly improved, 23 were unchanged, 3 were moderately worse, and 1 was markedly worse. – Odds of getting better instead of worst is 14.5:1 .

As a treatment, yes — some improves, many have no change, and more became worst than better. This does not seem like a rational choice – it’s playing craps with loaded dice.

It has been studies in terms or Crohn’s Disease, a more severe disruption of the microbiome,  “Currently, there is insufficient evidence to allow any firm conclusions regarding the efficacy and safety of LDN used to treat patients with active Crohn’s disease… is not without potential for side effects. Future use of methylnaltrexone may be better tolerated since it does not cross the blood brain barrier. ” [2014]

In terms of Fibromyalgia (FM) [2013], it appears to help — remember that FM is likely due to DNA factors — which suggest that its impact may be connected to this. Before taking it,  your  baseline erythrocyte sedimentation rate[ESR] should be taken to see if you are likely to be a responder, see table below.[2009]. Also, the effect decline within a week once you stop.

Like milnacipran, a related drug (which some suggest is better for FM), adverse effects can happen in 20-30% of patients [2009] “. “milnacipran 100 mg or 200 mg is effective for a minority in the treatment of pain due to fibromyalgia, providing moderate levels of pain relief (at least 30%) to about 40% of participants, compared with about 30% with placebo.” [2015]  So only 10% improved more than those with a placebo…

There is no clear pattern of ESR for IBS[2015], FM [2016]. It appears to apply to a subset only (likely DNA associated) which can be determined from the ESR. With CFS, browsing web pages you will find normal ESR is reported by some and low by others. One subset has low SED rates (hence LDN will likely have no positive effect for them)

• According to Dave Berg, Hemex (hypercoagulation theory):
  “The ESR (erythrocyte sedimentation rate – red blood cell sed rate) is called SED RATE for short. We are close to having enough data to publish that the normal range for SED RATES should start above 3 or 4. Values below this are correlated with high SFM values. As the Soluble Fibrin Monomer (SFM) goes up in the plasma, these molecules form dimers (2 stuck together). This physically blocks the RBCs from settling out of the plasma, thus a low sed rate.” [Townhall] – this is just ONE of the coagulation defects that he found in CFS. So some will be low and some normal and some high…
• This happens to be true for me with non-prescription items effective to lower SFM.

This leads to the possible mechanism of action, it’s does impact one form of anticoagulant!! There are many forms of coagulation. Coagulation reduces oxygen level to tissue, and this is well recognized as a major source of pain – cells screaming for oxygen.

• “The fibrinogen levels declined sharply with abstention and an additive effect was noted with the administration of naltrexone,” [1994]

Bottom Line

There is no clear evidence of it being effective with microbiome disruptions. Yes, a small percentage of patients improved (which many MD’s see emotionally and then start prescribing it to all of their CFS patients). Many CFS MDs may discount or ignore the patients that became worst (unfortunately) because they are focused on positive results instead of evaluation of this drug. With a non-fatal disease, a physician tossing a drug that is more likely to worsen a patient than improve the patient raises some  fundamental ethical questions.

If you have taken LDN and improved, don’t take exception with this post — I agree that you improved, the studies say that. You are the lucky ones…. others are not the same.

As a side note during my research I found “” With the exception of weak nonspecific DNA damage observed in an E. coli DNA repair test and possibly with WI-38 cells as well, Naltrexone did not demonstrate significant potential for the induction of gene mutations or chromosomal aberrations under the conditions of this evaluation.” indicating that there may be some long term risks. Damage to your microbiome DNA and your own DNA.

I work off a model which means that I know what needs to happen. It does not determine what the sequence is, or even fix the substances to use. This is actually good because what we are dealing with depends on three items: inherited DNA, inherited microbiome  (body bacteria, gut, mouth, etc) and likely the specific infection that triggered it.

Recently my perspective have shifted and this is my current suggestion for a new person kick-starting a run at remission. Since such a person likely has brain fog and a lack of executive decision making — I will restrict my self to 4 supplements (taken at the same time — no need to rotate, keep on them) and a list of 5 probiotics that should be taken in rotation. The rotation has two advantages — cuts cost, discourages resistance of the bad bacteria.

NOTE: Everything that is listed (with one exception) is documented as helping on PubMed National Institute of Health database of clinical studies. These are not reasoned recommendations, but recommendations based on actual human clinical study  experience that have been reviewed.

Supplements to mitigate symptoms

These are items that normally would be absorbed or transformed by a population of healthy bacteria. With the shift to the dark side, these are mal-absorbed or not produced to the same levels.

Having die-off / herx from these very rarely occurs.

Starting Probiotics

Today, we actually have an increasing number of suitable probiotics available over the internet. Most will not be found at your local health food store.

Probiotics are bacteria that often produce antibiotics against other species and strains. Whether you herx depends very much on the dysfunction bacteria that you have. If you have a low herx tolerance, then when a herx shows up — move along to the next one.

If a probiotic seem to help, fell free to continue for up to 2 more weeks (then you need to rotate it) and add in the next one on schedule (taking 2-3 concurrently). You will have to feel your way into these.

We do not know how well they play with each other. Some probiotics like Lactobacillus will not play nice with some of the above probiotics….

The above list delivers some 152 distinct named strains in the hope of kick starting bio-diversity that is very lacking in the FM/CFS/IBS gut.

I have some on order for my wife’s Crohn’s and will likely try some myself (since I am in remission, I do not expect significant effect).

A reader referred me to this product and he cites good result for himself. While there are no appropriate studies on it publish so far — it makes my theoretical good list. Why?

• No lactobacillus in it at all!
• A lot of species — with documentation on many in isolation

EU Buyers

Some readers have reported issues with customs and duties (or 3rd party charging outrageous rates). The direct European distributor is amitra nutrition in the UK.

Strains list

1. Bacterium 36B NCBI UniProt
2. Bacterium 72B NCBI UniProt
3. Bacterium AK-MB25 NCBI UniProt
4. Bacterium AK-MB30 NCBI UniProt
5. Bacterium AK-MB34 NCBI UniProt
6. Bacterium AK-MB37 NCBI UniProt
7. Bacterium BM0102 NCBI UniProt
8. Enterobacteriaceae bacterium BM0118 NCBI UniProt
9. Eubacteria bacterium BM0181 NCBI UniProt
10. Enterobacteriaceae bacterium BM0196 NCBI UniProt
11. Enterobacteriaceae bacterium BM0248 NCBI UniProt
12. Enterobacteriaceae bacterium BM0266 NCBI UniProt
13. Enterobacteriaceae bacterium BM0269 NCBI UniProt
14. Enterobacteriaceae bacterium BM0270 NCBI UniProt
15. Enterobacteriaceae bacterium BM0271 NCBI UniProt
16. Aeromonadaceae bacterium BM0272 NCBI UniProt
17. Enterobacteriaceae bacterium BM0277 NCBI UniProt
18. Enterobacteriaceae bacterium BM0287 NCBI UniProt
19. Enterobacteriaceae bacterium BM0294 NCBI UniProt
20. Pseudomonadaceae bacterium BM0295 NCBI UniProt
21. Enterobacteriaceae bacterium BM0298 NCBI UniProt
22. Enterobacteriaceae bacterium BM0301 NCBI UniProt
23. Enterobacteriaceae bacterium BM0305 NCBI UniProt
24. Enterobacteriaceae bacterium BM0315 NCBI UniProt
25. Aeromonadaceae bacterium BM0318 NCBI UniProt
26. Enterobacteriaceae bacterium BM0321 NCBI UniProt
27. Enterobacteriaceae bacterium BM0324 NCBI UniProt
28. Enterobacteriaceae bacterium BM0326 NCBI UniProt
29. Enterobacteriaceae bacterium BM0329 NCBI UniProt
30. Enterobacteriaceae bacterium BM0335 NCBI UniProt
31. Enterobacteriaceae bacterium BM0336 NCBI UniProt
32. Enterobacteriaceae bacterium BM0344 NCBI UniProt
33. Enterobacteriaceae bacterium BM0348 NCBI UniProt
34. Enterobacteriaceae bacterium BM0354_1 NCBI UniProt
35. Enterobacteriaceae bacterium BM0357 NCBI UniProt
36. Enterobacteriaceae bacterium BM0363 NCBI UniProt
37. Enterobacteriaceae bacterium BM0371 NCBI UniProt
38. Comamonadaceae bacterium BM0374 NCBI UniProt
39. Enterobacteriaceae bacterium BM0377 NCBI UniProt
40. Aeromonadaceae bacterium BM0380 NCBI UniProt
41. Enterobacteriaceae bacterium BM0381 NCBI UniProt
42. Enterobacteriaceae bacterium BM0382 NCBI UniProt
43. Enterobacteriaceae bacterium BM0383 NCBI UniProt
44. Aeromonadaceae bacterium BM0387 NCBI UniProt
45. Comamonadaceae bacterium BM0412 NCBI UniProt
46. Comamonadaceae bacterium BM0414 NCBI UniProt
47. Enterobacteriaceae bacterium BM0436 NCBI UniProt
48. Enterobacteriaceae bacterium BM0441 NCBI UniProt
49. Pseudomonadaceae bacterium BM0443 NCBI UniProt
50. Pseudomonadaceae bacterium BM0446 NCBI UniProt
51. Enterobacteriaceae bacterium BM0570 NCBI UniProt
52. Enterobacteriaceae bacterium BM0573 NCBI UniProt
53. Enterobacteriaceae bacterium BM0574 NCBI UniProt
54. Enterobacteriaceae bacterium BM0575 NCBI UniProt
55. Enterobacteriaceae bacterium BM0576 NCBI UniProt
56. Enterobacteriaceae bacterium BM0577 NCBI UniProt
57. Enterobacteriaceae bacterium BM0585 NCBI UniProt
58. Aeromonadaceae bacterium BM0587 NCBI UniProt
59. Enterobacteriaceae bacterium BM0595 NCBI UniProt
60. Enterobacteriaceae bacterium BM0602 NCBI UniProt
61. Enterobacteriaceae bacterium BM0603 NCBI UniProt
62. Enterobacteriaceae bacterium BM0609 NCBI UniProt
63. Enterobacteriaceae bacterium BM0610 NCBI UniProt
64. Bacterium KGJ-3-1 NCBI UniProt
65. Bacterium KGJ-3-10 NCBI UniProt
66. Bacterium KGJ-3-14 NCBI UniProt
67. Bacterium KGJ-3-16 NCBI UniProt
68. Bacterium KGJ-3-18 NCBI UniProt
69. Bacterium KGJ-3-22 NCBI UniProt
70. Bacterium KGJ-3-23 NCBI UniProt
71. Bacterium KGJ-3-5 NCBI UniProt
72. Bacterium KGJ-3-6 NCBI UniProt
73. Bacterium OC 16 NCBI UniProt
74. Bacterium AM_gF1DD01_08 NCBI UniProt
75. Bacterium AM_gF1DD01_14 NCBI UniProt
76. Bacterium AM_gF3SD01_02 NCBI UniProt
77. Bacterium AM_gF3SD01_03 NCBI UniProt
78. Bacterium AM_gF3SD01_04 NCBI UniProt
79. Bacterium AM_gF3SD01_06 NCBI UniProt
80. Bacterium AX_gF1DD01_04 NCBI UniProt
81. Bacterium AX_gF3SD01_11 NCBI UniProt
82. Bacterium AX_gF3SD01_12 NCBI UniProt
83. Bacterium AX_gF3SD01_17 NCBI UniProt
84. Bacterium AX_gF3SD01_25 NCBI UniProt
85. Bacterium AX_gF3SD01_29 NCBI UniProt
86. Bacterium AX_gF3SD01_33 NCBI UniProt
87. Bacterium AX_gF3SD01_39 NCBI UniProt
88. Bacterium AX_gF3SD01_48 NCBI UniProt
89. Bacterium CA_gF2-3DD01_02 NCBI UniProt
90. Bacterium CA_gF2-3DD01_03 NCBI UniProt
91. Bacterium CA_gF3SD01_04 NCBI UniProt
92. Bacterium CA_gF3SD01_10 NCBI UniProt
93. Bacterium CY_gF1DD01_05 NCBI UniProt
94. Bacterium CY_gF1DD01_09 NCBI UniProt
95. Bacterium CY_gF1DD01_10 NCBI UniProt
96. Bacterium CY_gF1DD01_14 NCBI UniProt
97. Bacterium PE_gF1DD01_04 NCBI UniProt
98. Bacterium PE_gF1DD01_06 NCBI UniProt
99. Bacterium PE_gF1DD01_11 NCBI UniProt
100. Bacterium PE_gF3SD01_05 NCBI UniProt
101. Bacterium PI_gF3SD01_19 NCBI UniProt
102. Bacterium A1Q1_fos_2300 NCBI UniProt
103. Pseudomonadales bacterium HF0500_12O04 NCBI UniProt
104. Bacterium HF130_AEPn_2 NCBI UniProt
105. Bacterium HH1107 NCBI UniProt
106. Bacterium HHV216 NCBI UniProt
107. Bacterium HHV35 NCBI UniProt
108. Bacterium MID12 NCBI UniProt
109. Bacterium pAB2 NCBI UniProt
110. Bacterium A1Q1_fos_2300 NCBI UniProt
111. Bacterium HF130_AEPn_2 NCBI UniProt
112. Pseudomonas sp. R2SpM1P1C10 NCBI UniProt – also eats mustard gas!
113. Pseudomonas sp. UMAB-62 NCBI UniProt
114. Pseudomonas sp. cn4902 NCBI UniProt
115. Pseudomonas stutzeri NCBI UniProt – NCBI study

You can order it directly from the manufacturer, and they ship to Europe for just an extra $3.00!!!

This is the kicker — full refund if you are not happy!!!!!

“We want to make you happy. Try Equilibrium and see if you like it. If you do, awesome! If you don’t, just email us and we’ll refund you completely. Max 10 bottles refunded per person, within 180 days of purchase.” — also applies to international orders!

If you are new to probiotics, I would suggest one capsule of the above at breakfast and one of Prescript Assist at bedtime snack — some 150 species (and not a single lactobacillus in either!)

The have a good, well documented read, at http://www.generalbiotics.com/science/

Experiences

• ” I was hoping to see noticable improvement and relief of IBS symptoms. I would say while on this product I noticed UP TO 20% benefit/improvement from this product.” [Source]
• “I found this to be the most effective digestive aid that I’ve used to date. All the typical digestive signs were present – more regular bowel movements, no bloating or gas, and overall just a more normal sense of fullness and digestion between meals. While most of this is anecdotal, I do firmly believe that this proved more effective at over-all wellness than other probiotics that seemed to simply flush my system and make me fart alot.” [Source]
• ” Equilibrium is at the top of the price range. This could quite easily be a ‘you get what you pay for’ scenario however, as I mentioned above, no other probiotic has worked for me like this one.” [Source]