An ideal FMT Donor scenario for ME/CFS

A reader pointed me to this blog, our2ndbrain.com. He did a DYI FMT transplant using a donation from his daughter [post] and obtained remission.

” For many of you visiting this site, you may have wondered why I picked my daughter as the donor for Fecal Microbiota Transplant.   “

Kudos!

I say kudos because this is a very ideal choice of donor! (on the matter of DYI Fmt, I choose, for legal reasons, to keep mute). The whys are simple:

  • As close to the same DNA as possible (50%)
  • Same diet (which means that the bacteria mix has already been tuned to the diet)
  • A son could technically be better (since gender is a factor for the microbiome)
  • Same longitude (which is a factor)
  • Younger microbiome — which usually means stronger and more robust. The microbiome “has not slipped into old age”

Bottom Line

I am not advocating people to volunteer to change diapers of their kids and grandkids to get material for DYI FMT. I am advocating, if you have kids (if you do not have kids, if a sibling has children those are better candidates than a random person IMHO), and have a MD willing to go down the FMT path, to advocate for those people as donors, instead of doing the DYI approach on this site.

The following paper gives some background on FMT. There is still disagreement whether family members give better results ( I suspect those studies were done on siblings and not descendents).

THE POWER OF POOP: FECAL MICROBIOTA TRANSPLANTATION FOR CLOSTRIDIUM DIFFICILE INFECTION

Drug induced histamine issues

I came across an interest article on enteral-induced histaminosis (Histaminosis: Studies on Three Prognostic Variables in an Epidemiological Model) which identifies some drugs that inhibit DAO production.

This lead me to this page which gives a fuller list with citations.

Active substanceExamples of products ®CategoriesHistamine effectsReferences
Acemetacin AntirheumaticDAO inhibitor[Sattler 1985, Fritzsche 2009]
Acetyl­cysteineFluimucil, Helvetussin, Muco-Mepha, NeoCitran, SolmucolMucolytic, antidoteDAO inhibitor[Jarisch 2004, Maintz et al. 2006]
Acriflavin AntisepticDAO inhibitor[Fritzsche 2009]
Alcuronium Muscle relaxantDAO inhibitor[Sattler 1985, Maintz et al. 2006, Forth 2008]
Alprenolol Beta blockerDAO inhibitor[Maintz et al. 2006]
AmbroxolAmbrovene, Ambroxol, Broxol, Mucosolvan, MucospasExpectorantDAO inhibitor[Jarisch 2004, Maintz et al. 2006]
Aminocycline  DAO inhibitor[Sattler 1985]
AminophyllinEuphyllin, Mundiphyllin, MyocardonAntiasthmaticDAO inhibitor[Sattler 1985, Jarisch 2004, Maintz et al. 2006]
Amiphenazole  DAO inhibitor[Sattler 1985]
AmitriptylineSaroten, Tryptizol, LimbritolTricyclic antidepressantDAO inhibitor[Jarisch 2004, Maintz et al. 2006]
Carbocromene  DAO inhibitor[Sattler 1985]
Cefotiam AntibioticDAO inhibitor[Maintz et al. 2006]
Cimetidine H2 antihistamineDAO inhibitor[Prof. Ralf Bauer Uni Bonn; found in: Jarisch 2004 S.12, Maintz et al. 2006, Fritzsche 2009]
Clavulanic acidAugmentinAntibioticDAO inhibitor[Sattler 1985, Jarisch 2004, Maintz et al. 2006]
Colistin mesilate  DAO inhibitor[Sattler 1985]
DiazepamValiumTranquilizerDAO inhibitor[Fritzsche 2009]
DihydralazineNepresolAntihypertensive, vasodilatorDAO inhibitor[Wantke et al. 1989, Sattler 1985, Maintz et al. 2006, Fritzsche 2009]
Fenpiverinium  DAO inhibitor[Sattler 1985]
Framycetin AntibioticDAO inhibitor[Sattler 1985, Fritzsche 2009]
FurosemideLasixDiureticDAO inhibitor[Fritzsche 2009]
HaloperidolHaldolNeurolepticDAO inhibitor[Fritzsche 2009]
Metamizole, dipyronesNovalgin, MinalginAnalgesic, antipyreticDAO inhibitor[Jarisch 2004, Maintz et al. 2006]
MetoclopramideMigpriv, Paspertin, PrimperanAntiemetic, gastroenterologic, dopamine antagonistDAO inhibitor[Sattler 1985, Jarisch 2004, Maintz et al. 2006]
Neomycin AntibioticDAO inhibitor[Mathelier-Fusade 2006]
Novamine sulfone(=Metamizol) Novalgin, MinalginAnalgesic, antipyreticDAO inhibitor[Jarisch 2004]
Orciprenaline  DAO inhibitor[Sattler 1985]
Pancuronium Muscle relaxantDAO inhibitor[Sattler 1985, Maintz et al. 2006, Fritzsche 2009]
Pentamidine AntibioticDAO inhibitor[Sattler 1985, Maintz et al. 2006]
Pirenzepine  DAO inhibitor[Sattler 1985]
Prilocaine Local anaestheticDAO inhibitor[Maintz et al. 2006, Nesterenko 2010]
PromethazineAtosil, Closin, Proneurin, ProthazinSedative, antihistamine, antipsychoticDAO inhibitor[Irion 2009]
PropafenoneRytmonormAntiarrhythmicDAO inhibitor[Jarisch 2004, Maintz et al. 2006]
Propanidide AnaestheticDAO inhibitor[Nesterenko 2010]
Quinidine CardiacDAO inhibitor[Fritzsche 2009]
Tetroxoprim  DAO inhibitor[Sattler 1985]
VerapamilFlamon, Isoptin, TarkaCoronaryvasodilatant, antihypertensive, antiarrhythmic, calcium antagonistDAO inhibitor[Jarisch 2004, Maintz et al. 2006]
D-CycloserineSeromycinAntibioticDAO inhibitor (Vitamin B6-Antagonist)[Sattler 1985, Steneberg 2007]
IsoniazideRimifon, RifaterTuberculostaticDAO inhibitor (Vitamin B6-Antagonist)[Jarisch 2004, Maintz et al. 2006]
ChloroquineChlorochin, Nivaquine, ResochinAntimalarials, antirheumaticDAO inhibitor, HNMT-Blocker[Sattler 1985, Jarisch 2004, Donatelli et al. 1994, Maintz et al. 2006]

Bottom Line

The list are items that you should NOT take because they reduce DAO and can result in fatal histamine reactions if you already have histamine issues. Decision to stop any should be done in consultation with your medical professional.

Ajwain (Trachyspermum Ammi)

“No good deed goes unpunished” – as a result of my last post on Sumac, I was asked about another atypical herb. It is a herb that has been used in cooking for a long time: Ajwain, ajowan caraway, bishop’s weed, carom, Ptychotis Oil, Ethiopian Cumin,Omam etc.

I was actually an active user of it some 20 years ago for it’s anticoagulation effects.

Trachyspermum ammi commonly known as ‘Ajwain’ is distributed throughout India and is mostly cultivated in Gujarat and Rajasthan. The fruit possesses stimulant, antispasmodic and carminative properties and is used traditionally as an important remedial agent for flatulence, atonic dyspepsia, diarrhea, abdominal tumors, abdominal pains, piles, and bronchial problems, lack of appetite, galactogogue, asthma and amenorrhoea. Medicinally, has been proven to possess various pharmacological activities like antifungal, antioxidant, antimicrobial, antinociceptive, cytotoxic, hypolipidemic, antihypertensive, antispasmodic, broncho-dilating actions, antilithiasis, diuretic, abortifacient, antitussive, nematicidal, anthelmintic and antifilarial. Further, studies reveal the presence of various phytochemical constituents mainly carbohydrates, glycosides, saponins, phenolic compounds, volatile oil (thymol, γ-terpinene, para-cymene, and α- and β-pinene), protein, fat, fiber and mineral matter containing calcium, phosphorous, iron and nicotinic acid. These studies reveal that T. ammi is a source of medicinally active compounds and have various pharmacological effects; hence, it is encouraging to find its new therapeutic uses.

Trachyspermum ammi [2012] – Full text with links to studies

The major component of this seed is thymol which impacts hundreds of different bacteria. It has a higher concentration than thyme.

Handling non 16s Tests

Today I added another tests to the list on this page. This is the 2nd one from this lab.

Dealing with non 16s tests means that a totally different lab process is used with ranges being different. Ranges for most labs is done by taking a sample of people close to the labs and then computing average and standard deviations. This works fine if you are a match for the people in the sample (DNA, diet, etc.) If you are Hindu vegetarian working in pork-country of Germany — your numbers may be very different. The reality is that their values are the only thing we can work from.

The lab may show values like this, with the ranges covering 65-95% of the population according to statistics. The numbers from our 16s samples do not agree. Instead of 5% being outside of the normal range, we could have 80% outside.

1.5 occurs at the 60%ile. 40% are higher.
The labs’ range is 25%-47%, 80% would be abnormal

Each lab will have different normal ranges. The solution that I used to allow people to use the suggestion engine is pretty simple.

  • Red below – pick two down arrows
  • Yellow below – pick one down arrows
  • Green — no errors
  • Yellow above – pick one up arrows
  • Red above – pick two up arrows
The ( %) can be ignored… we really just use the direction.

Can you use Jason Harwelak reference ranges? In general, likely. The values are below, so just copy your numbers across. I used the results from the above A6 test below. Notice that there can be major disagreement between the two on what the desired range is!!!

TaxonomyRankLowHighYour ValueStatus
Bacteroidetes
A6: 27 – 36
class03539.7Not Ideal
Akkermansia
A6: 0.01-1.5
genus135.2Not Ideal
Bacteroides
A6: 12-15
genus02013.2Not Ideal
Bifidobacterium
A6: 0.6 – 4.5
genus2.550.12Not Ideal
Blautiagenus510MISSING
Desulfovibrio
A6: 0 – 0.1
genus00.250.026Ideal
Eubacterium
A6: 0.3 – 2.3
genus0150.342Ideal
Lactobacillus
A6: 0.01 -0.05
genus0.0110.012Ideal
Methanobrevibacter
A6: 0 – 0.03
genus0.00010.020Ideal
Roseburia
A6: 0.5 – 2.4
genus5100.145Not Ideal
Ruminococcus
A6; 4.9 – 8.1
genus0153.9 Ideal
Proteobacteria
A6: 2-5
phylum049.5Not Ideal
Bilophila wadsworthia
A6: 0 – 1.89
species00.250.450Not Ideal
Escherichia colispecies00.01MISSING
Faecalibacterium prausnitzii
A6: 1.9 – 5.0
species10152.986Not Ideal

Remember, on the data entry — you can pick if you want to deem a value to be normal or abnormal. No one has the perfect answer (unfortunately).

Bottom Line

Try to isolate definite issues.

  • If the lab says “normal” skip that bacteria
  • If it says outside of range a little, you may wish to skip it.
  • If it says outside a lot,
    • Check Jason’s ranges
    • Click on the bacteria link and see if your values are:
      • In the top 5-10% of values
      • In the bottom 5% for bacteria that 99% of people have

Then click Analysis and see what suggestions comes up. If the lab has suggestions, compare them. If both agree — those are your first choices. If one is quiet and the other recommends, those are your second choices.

If the suggestions disagree — discuss with your medical professional before doing.

All of the tests below follow the same pattern:

Sumac – Rhus coriaria

A reader ask me about this and a quick browse found that it definitely warrants a post because it is off the usual beaten path for both conventional and alternative medicine. It’s a herb that you may wish to season your meals with. It has a nice profile.

Rhus coriaria L. (Anacardiaceae), known as sumac, is a perennial edible plant, which grows wild in Aegean, Mediterranean, Southeast, Central and Northern regions of Turkey. [2019]

” Anti-inflammatory, antioxidant, immunomodulatory, and anti-apoptotic activities of Sumac(RC) are especially due to its phenolic compounds. In this study, the anti-inflammatory, antioxidant, immunomodulatory, and anti-apoptotic activities of RC were demonstrated in a rat NEC model. RC can suggest as a new treatment option for preventing intestinal injury. ” [2019]

” Gallic acid was determined as the primary phenolic acid in the extracts of R. coriaria, followed by cyanidin, peonidin, pelargonidin, petunidin, delphinidin glucosides and coumarates. R. coriaria also contains some organic acids including malic acid, citric acid, tartaric acid and fumaric acid ” [2019]

The ethanol extract of the fruit of the genetically related Rhus coriaria L., known as sumac, afforded protocatechuic acid, isoquercitrin, and myricetin-3-O-α-L-rhamnoside from the fruits for the first time, in addition to the previously reported phenol acids and flavonoids, gallic acid, methyl gallate, kaempferol, and quercetin. [2011]

Reported in Studies to reduce the following

Actinomyces viscosus
Bacillus cereus
Bacillus megaterium
Bacillus subtilis
Bacillus thuringiensis
Citrobacter freundii
Enterococcus faecalis
Escherichia coli
Hafnia alvei
Lactobacillus rhamnosus
Proteus vulgaris
Staphylococcus aureus
Streptococcus mutans
Streptococcus salivarius
Streptococcus sanguinis
Streptococcus sobrinus

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