Handling non 16s Tests

Today I added another tests to the list on this page. This is the 2nd one from this lab.

Dealing with non 16s tests means that a totally different lab process is used with ranges being different. Ranges for most labs is done by taking a sample of people close to the labs and then computing average and standard deviations. This works fine if you are a match for the people in the sample (DNA, diet, etc.) If you are Hindu vegetarian working in pork-country of Germany — your numbers may be very different. The reality is that their values are the only thing we can work from.

The lab may show values like this, with the ranges covering 65-95% of the population according to statistics. The numbers from our 16s samples do not agree. Instead of 5% being outside of the normal range, we could have 80% outside.

1.5 occurs at the 60%ile. 40% are higher.
The labs’ range is 25%-47%, 80% would be abnormal

Each lab will have different normal ranges. The solution that I used to allow people to use the suggestion engine is pretty simple.

  • Red below – pick two down arrows
  • Yellow below – pick one down arrows
  • Green — no errors
  • Yellow above – pick one up arrows
  • Red above – pick two up arrows
The ( %) can be ignored… we really just use the direction.

Can you use Jason Harwelak reference ranges? In general, likely. The values are below, so just copy your numbers across. I used the results from the above A6 test below. Notice that there can be major disagreement between the two on what the desired range is!!!

TaxonomyRankLowHighYour ValueStatus
Bacteroidetes
A6: 27 – 36
class03539.7Not Ideal
Akkermansia
A6: 0.01-1.5
genus135.2Not Ideal
Bacteroides
A6: 12-15
genus02013.2Not Ideal
Bifidobacterium
A6: 0.6 – 4.5
genus2.550.12Not Ideal
Blautiagenus510MISSING
Desulfovibrio
A6: 0 – 0.1
genus00.250.026Ideal
Eubacterium
A6: 0.3 – 2.3
genus0150.342Ideal
Lactobacillus
A6: 0.01 -0.05
genus0.0110.012Ideal
Methanobrevibacter
A6: 0 – 0.03
genus0.00010.020Ideal
Roseburia
A6: 0.5 – 2.4
genus5100.145Not Ideal
Ruminococcus
A6; 4.9 – 8.1
genus0153.9 Ideal
Proteobacteria
A6: 2-5
phylum049.5Not Ideal
Bilophila wadsworthia
A6: 0 – 1.89
species00.250.450Not Ideal
Escherichia colispecies00.01MISSING
Faecalibacterium prausnitzii
A6: 1.9 – 5.0
species10152.986Not Ideal

Remember, on the data entry — you can pick if you want to deem a value to be normal or abnormal. No one has the perfect answer (unfortunately).

Bottom Line

Try to isolate definite issues.

  • If the lab says “normal” skip that bacteria
  • If it says outside of range a little, you may wish to skip it.
  • If it says outside a lot,
    • Check Jason’s ranges
    • Click on the bacteria link and see if your values are:
      • In the top 5-10% of values
      • In the bottom 5% for bacteria that 99% of people have

Then click Analysis and see what suggestions comes up. If the lab has suggestions, compare them. If both agree — those are your first choices. If one is quiet and the other recommends, those are your second choices.

If the suggestions disagree — discuss with your medical professional before doing.

All of the tests below follow the same pattern:

Sumac – Rhus coriaria

A reader ask me about this and a quick browse found that it definitely warrants a post because it is off the usual beaten path for both conventional and alternative medicine. It’s a herb that you may wish to season your meals with. It has a nice profile.

Rhus coriaria L. (Anacardiaceae), known as sumac, is a perennial edible plant, which grows wild in Aegean, Mediterranean, Southeast, Central and Northern regions of Turkey. [2019]

” Anti-inflammatory, antioxidant, immunomodulatory, and anti-apoptotic activities of Sumac(RC) are especially due to its phenolic compounds. In this study, the anti-inflammatory, antioxidant, immunomodulatory, and anti-apoptotic activities of RC were demonstrated in a rat NEC model. RC can suggest as a new treatment option for preventing intestinal injury. ” [2019]

” Gallic acid was determined as the primary phenolic acid in the extracts of R. coriaria, followed by cyanidin, peonidin, pelargonidin, petunidin, delphinidin glucosides and coumarates. R. coriaria also contains some organic acids including malic acid, citric acid, tartaric acid and fumaric acid ” [2019]

The ethanol extract of the fruit of the genetically related Rhus coriaria L., known as sumac, afforded protocatechuic acid, isoquercitrin, and myricetin-3-O-α-L-rhamnoside from the fruits for the first time, in addition to the previously reported phenol acids and flavonoids, gallic acid, methyl gallate, kaempferol, and quercetin. [2011]

Reported in Studies to reduce the following

Actinomyces viscosus
Bacillus cereus
Bacillus megaterium
Bacillus subtilis
Bacillus thuringiensis
Citrobacter freundii
Enterococcus faecalis
Escherichia coli
Hafnia alvei
Lactobacillus rhamnosus
Proteus vulgaris
Staphylococcus aureus
Streptococcus mutans
Streptococcus salivarius
Streptococcus sanguinis
Streptococcus sobrinus

Sumac Spice Powder | 15 oz - 425 g Reseable Bag | Bulk Ground Sumac Berries - Bran |Extra Grade Turkish Sumac Seasoning | Middle Eastern Spices | by Eat Well Premium Foods

Night Sweats

I have briefly touched upon night sweats in an earlier post, Biohacking Night Sweats and though it may be good to revisit the little literature on it. It is a symptoms of a vast multitude of conditions. Many of the conditions have association with some form of microbiome dysfunction.

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Diversity, stability and resilience of the human gut microbiota [2012]
” A rise above the optimum induces thermoregulatory mechanisms such as sweating to reduce it, and a descent below the optimum induces mechanisms such as shivering to increase it.  “
top 3 causes of night sweats
https://www.clearvuehealth.com/b/nightsweatbasics/

A microbiome dysfunction seems the most common cause with the bacteria involved likely producing more than normal amount of energy.

Night sweats are repeated episodes of extreme perspiration that may soak your nightclothes or bedding and are related to an underlying medical condition or illness.

Mayo Clinic – really helpful NOT,

Citizen Science?

Using contributed data and microbiomes we found these statistically significant:

Clearly, Barley with walnuts for breakfast is recommended. Licorice and Berberine also. For the latter two, inference suggests it may help

Bottom Line

In terms of formal science, we get no traction. In terms of citizen science, we came up with some concrete suggestions that are very low risk.

Encourage others

This is the start of a new year and new decade. I am a data consolidator not a physician or medical person. I am an Expedia or Travelocity not a KLM, Delta or Air India. If the information on this site or http://microbiomeprescription.com/ has had a positive effect on you, consider adding your experience as a comment. Be specific and try giving the following information:

  • What data did you base that decision on? (Thryve, uBiome, GI Map)
    • The specific condition/symptom that you were attempting to address
  • What did you try (including brand and web link)
    • If this came from a specific post or page, include it
  • When was the positive outcome or change (i.e. how long to see changes)

The purpose is to give encouragement and hope to others struggling with issues.

Thanks — and have a good new year on this 8th day of Twelveth Night!

Microbiome and Iron, Calcium, Phosphate, Zinc…

However, Bifidobacteriaceae are capable of binding iron in the large intestine, thereby limiting the formation of free radicals synthesized in the presence of iron, and thus reducing the risk of colorectal cancer. Animal studies have revealed that supplementation with probiotics, prebiotics and synbiotics has a significant effect on bone calcium, phosphate and bone metabolism. The dynamic interaction between microbiota and zinc was shown. Human studies have provided evidence of the influence of probiotic bacteria on parathormone, calcium and phosphate levels and thus on bone resorption. Recent studies have produced new information mainly on the impact of the intestinal bacteria on the metabolism of calcium and iron. From a scientific perspective, the most urgent fields that remain to be investigated are the identification of all human gut microbes and new therapies targeting the interaction between intestinal bacteria and minerals.

Association between the gut microbiota and mineral metabolism. [2018]

In this post, I will try finding the studies and further information.

Bottom Line

The available literature suggests that Lactobacillus and Bifidobacterium are dominate player for calcium levels. Since these are much higher in children than adults, this would assist in bone growth by providing the needed calcium. This appears to extend to other minerals such as zinc, copper, phosphorus in a similar manner.

Both Lactobacillus and Bifidobacterium decrease with old age and may account for calcium /bone loss issues. ” The presence of the BifidobacteriumFaecalibacteriumBacteroides group, and Clostridium cluster XIVa decreased with age up to 66-80 years of age,  “[2019]

Low levels of one of more of theses would hint towards insufficient of these, or a bad balance with them. High levels of one or more would hint towards a surplus of these, or a bad balance with them.