We have now exploded to 53 items to review when we examine the “Family Level” of bacteria in the gut. Our decomposition may reveal that while a higher Order is normal, specific families are not (usually underpopulated).

A few items are close enough to normal by one or another criteria that I will skip them entirely. Definition for normal that I used was 5 or more values in the 60% -166% range with middle value in the same range):

• Acidaminococcaceae: Middle value 73%
• Bacteroidaceae: Middle value 104%
• Clostridiaceae: Middle value 95%
• Coriobacteriaceae: Middle value 60%
• Erysipelotrichaceae: Middle value 67%
• Oscillospiraceae: Middle value 67%
• Peptostreptococcaceae: Middle value 61%
• Porphyromonadaceae: Middle value 86%
• Verrucomicrobiaceae: Middle value 105%

While there may be some significant, the magnitude of this significance is much less than the items below

Massive Shifts

Criteria is at least 11 values with 5 or less in the range (60% – 160%) and the middle value is outside of the range

• Lactobacillaceae  [ Firmicutes / Bacilli / Lactobacillales ]:
  • Middle Value 17%, in above range 2
• Bifidobacteriaceae [  Actinobacteria / Actinobacteridae / Bifidobacteriales ]:
  • Middle Value 5%, in above range 2
• Enterobacteriaceae [Proteobacteria  / Gammaproteobacteria / Enterobacteriales ]:
  • Middle Value 18%, in above range 2
• Peptococcaceae [Firmicutes  / Clostridia / Clostridiales]:
  • Middle Value 11%, none in above range
• Streptococcaceae [Firmicutes / Bacilli / Lactobacillales]:
  • Middle Value 17%, in above range 5
• Veillonellaceae [Firmicutes / Negativicutes / Selenomonadales]:
  • Middle Value 15%, in above range 5
  • “other species being representatives of several human and other animal microbiotae, and some of them can act as opportunistic pathogens for animals including humans being usually responsible for polymicrobial infections and more rarely for monomicrobial severe infections like osteoarticular infections or endocarditis.”

Not Enough Information

If at least three different people have a family of bacteria, I deem it to be noise. It may apply to the person and their symptoms but is not revelant to a general CFS patient.

Possible Symptom Specific Shifts

Must be a clear pattern (i.e. at least 70% very low) and at least 3 results

• Actinomycetaceae  [Actinobacteria / Actinobacteria / Actinomycetales ]
• Catabacteriaceae [Firmicutes / Clostridia / Clostridiales ]
• Clostridiales Family XI. Incertae Sedis [Firmicutes / Clostridia / Clostridiales]
• Clostridiales Family XIII. Incertae Sedis  [Firmicutes / Clostridia / Clostridiales]
• Enterococcaceae [ Firmicutes / Bacilli / Lactobacillales   ]
• Eubacteriaceae [Firmicutes / Clostridia / Clostridiales]
• Oxalobacteraceae [Proteobacteria / Betaproteobacteria/ Burkholderiales]
• Pasteurellaceae [ Proteobacteria / Gammaproteobacteria / Pasteurellaceae]
• Prevotellaceae [Bacteroidetes / Bacteroidetes/ Bacteroidales]
• Thermoanaerobacteraceae [ Firmicutes / Clostridia/ Thermoanaerobacterales ]

Analysis

In looking at various families above, I noted a large number of them were first “discovered” in the last 20 years. They were discovered by modern lab methods such as real-time polymerase chain reaction (PCR) that amplifies RNA and DNA.  The downside of this is simple: we can identify the bacteria but we know very little about what is associated or caused with each. We know even less about how to correct shifts. Modern medicine knows how to treat overpopulation of bacteria (antibiotics) but really nothing beyond “eat yogurt”  for dealing with underpopulation — what is clearly seen above.

For four of the massive shift items, we have probiotics available in the world. The problem is that many species do not play well with other species. In terms of correction (in sequence), my best suggestions (which does evolve):

1. High dosages of different Bifidobacteriaceae, rotating thru as many species as possible. We saw with results C that it does work to shift bacteria. The problem is their failing to take up residence.
2.  Enterobacteriaceae probiotics — that is E.Coli probiotics from Germany (Mutaflor, Symbioflor-2) – the latter is known to take up residence.
   1. Should be taken with Enterococcaceae probiotic since they appear to support each other. E. faecium SF68, etc
3. D-Lactic acid free and H2O2 producing Lactobacillaceae probiotics
4. Streptococcaceae probiotics, for example Streptococcus salivarius K12

Downloads

Pdf: family

Excel: family